mouse anti-human icam-2  (Thermo Fisher)

Journal: Mediators of Inflammation

Article Title: Targeting Endothelial Adhesion Molecule Transcription for Treatment of Inflammatory Disease: A Proof-of-Concept Study

doi: 10.1155/2016/7945848

Figure Lengend Snippet: Graphs showing relative ICAM-1 transcript expression in human retinal endothelial cell monolayers, following stimulation with TNF- α for 60 minutes or 24 hours, as determined by qRT-PCR using two ICAM-1 primer pairs. Reference gene was 18S rRNA. The same result was obtained when data were normalized to cyclophilin A reference gene. (a) shows the result obtained using ICAM-1 primer pair listed in that yields 282 bp product. (b) shows similar result obtained using ICAM-1 primer pair listed in that yields 218 bp product. Bars represent mean relative expression, with error bars showing standard error of the mean. n = 5 monolayers/condition. Data were analyzed by two-tailed Student's t -test. Human retinal endothelial RNA samples used for this experiment were used for PCR array profiling study presented in .

Article Snippet: Mouse monoclonal anti-human ICAM-1 antibody (clone LB-2, isotype IgG 2b, κ ) and mouse monoclonal IgG (clone 27-35, isotype IgG 2b, κ ) were obtained from BD Pharmingen (San Jose, CA).

Techniques: Expressing, Quantitative RT-PCR, Two Tailed Test

Journal: Mediators of Inflammation

Article Title: Targeting Endothelial Adhesion Molecule Transcription for Treatment of Inflammatory Disease: A Proof-of-Concept Study

doi: 10.1155/2016/7945848

Figure Lengend Snippet: Transcription factors with putative binding sites within the regulatory region of intercellular adhesion molecule 1 ( ICAM-1 ) that increase in human retinal endothelial cells treated with tumor necrosis factor-alpha (TNF- α ). For the purposes of this analysis, the ICAM-1 regulatory region was defined as 5000 bp of sequence immediately upstream of the ICAM-1 gene transcription start site and the first 2 introns. The ICAM-1 gene sequence was interrogated in the JASPAR database [ 21 ].

Article Snippet: Mouse monoclonal anti-human ICAM-1 antibody (clone LB-2, isotype IgG 2b, κ ) and mouse monoclonal IgG (clone 27-35, isotype IgG 2b, κ ) were obtained from BD Pharmingen (San Jose, CA).

Techniques: Binding Assay, Sequencing

Journal: Mediators of Inflammation

Article Title: Targeting Endothelial Adhesion Molecule Transcription for Treatment of Inflammatory Disease: A Proof-of-Concept Study

doi: 10.1155/2016/7945848

Figure Lengend Snippet: Sequences and locations of NF- κ B1 putative binding sites within the regulatory region of intercellular adhesion molecule 1 ( ICAM-1 ). For the purposes of this study, the ICAM-1 regulatory region was defined as 5000 bp of sequence immediately upstream of the ICAM-1 gene transcription start site and the first 2 introns. Gene sequences were obtained from the JASPAR database [ 21 ].

Article Snippet: Mouse monoclonal anti-human ICAM-1 antibody (clone LB-2, isotype IgG 2b, κ ) and mouse monoclonal IgG (clone 27-35, isotype IgG 2b, κ ) were obtained from BD Pharmingen (San Jose, CA).

Techniques: Binding Assay, Sequencing

Journal: Mediators of Inflammation

Article Title: Targeting Endothelial Adhesion Molecule Transcription for Treatment of Inflammatory Disease: A Proof-of-Concept Study

doi: 10.1155/2016/7945848

Figure Lengend Snippet: TNF- α -induced expression of cellular ICAM-1 is significantly reduced by NF- κ B1 silencing, but constitutive expression is not impacted. Human retinal endothelial cell monolayers were transfected with NF- κ B1-targeted (NF- κ B1) siRNA or nontargeted (NT) control siRNA and treated with TNF- α or no cytokine for 24 hours. Cellular ICAM-1 was fluorescently labeled by indirect immunocytochemistry; fluorescence of endothelial monolayers was read by microplate reader and adjusted for background fluorescence and cell number. (a) Graph showing relative level of ICAM-1 protein on the surface of endothelial cells. Bars represent mean relative level, with error bars showing standard error of the mean. n = 8 monolayers/condition. Data were analyzed by two-tailed Student's t -test. ns = not significant. (b) Graph showing relative NF- κ B1 transcript expression in human retinal endothelial cell monolayers that were transfected in parallel. Reference gene was 18S rRNA. Bars represent mean relative expression, with error bars showing standard error of the mean. n = 4 monolayers/condition. Data were analyzed by two-tailed Student's t -test.

Article Snippet: Mouse monoclonal anti-human ICAM-1 antibody (clone LB-2, isotype IgG 2b, κ ) and mouse monoclonal IgG (clone 27-35, isotype IgG 2b, κ ) were obtained from BD Pharmingen (San Jose, CA).

Techniques: Expressing, Transfection, Labeling, Immunocytochemistry, Fluorescence, Two Tailed Test